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. 2018 Dec 12;47(4):2056–2074. doi: 10.1093/nar/gky1241

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Respiration assays. (A) Analysis of O₂ consumption in the various cell lines using different inhibitors. The rates of oxygen consumption (OCR) were first measured on 2 × 10⁴ cells of each cell line under basal conditions and then with sequential additions of oligomycin (1.5 μM), FCCP (0.8 μM), rotenone (3.0 μM), and antimycin A (1.5 μM) at the indicated times to determine different parameters of mitochondrial functions. (B) Graphs presented the basal OCR, ATP-linked OCR, proton leak OCR, maximal OCR, reserve capacity OCR and non-mitochondrial OCR in HUVEC, mutant and control cybrid cell lines. Basal OCR was determined as OCR before oligomycin minus OCR after rotenone/antimycin A. ATP-linked OCR was determined as OCR before oligomycin minus OCR after oligomycin. Proton leak OCR was determined as basal OCR minus ATP-linked OCR. Maximal OCR was determined as the OCR after FCCP minus non-mitochondrial OCR. Reserve capacity OCR was defined as the difference between maximal OCR after FCCP minus basal OCR. Non-mitochondrial OCR was determined as the OCR after rotenone/antimycin A treatment. The average of 6 determinations for each cell line is shown. Graph details and symbols are explained in the legend to Figure 3.