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. 2018 Dec 12;47(4):2056–2074. doi: 10.1093/nar/gky1241

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — Mitochondrial membrane potential analysis. The mitochondrial membrane potential (ΔΨm) was measured in mutant and control cybrid cell lines by BD-LSR II flow cytometer system using a fluorescence probe JC-10 assay system. The ratio of the Ex/Em = 488/590 nm and 488/525 nm fluorescence intensities (FL590/FL525) was recorded to determine the ΔΨm level of each sample. Flow cytometry images of H25-03 and IV-3-13 without (A) and with (C) FCCP. Relative ratio of JC-10 fluorescence intensities at Ex/Em = 490/530 nm and 490/590 nm in (B) absence and (D) presence of 10 μM of FCCP. Graph details and symbols are explained in the legend to Figure 3.