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. 2018 Dec 12;47(4):2056–2074. doi: 10.1093/nar/gky1241

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 9. — Measurement of mitochondrial ROS. Ratio of geometric mean intensity between levels of the ROS generation in the vital cells with or without H₂O₂ stimulation. The rates of mitochondrial ROS production in three mutant cybrid cell lines, three control cell lines and HUVECs, were analyzed by BD-LSR II flow cytometer system using MitoSOX Red Mitochondrial Superoxide Indicator. Flow cytometry histogram showing MitoSOX-Red fluorescence of H25-03 (red) and IV-3-13 (green) without (A) or with (C) H₂O₂ stimulation. The relative ratios of fluorescence intensity (stimulated versus unstimulated with H₂O₂) was calculated: (B) and (D). Graph details and symbols are explained in the legend to Figure 3.