Figure 4.
DNA synthesis is required for a DNA polymerase to stabilize strand exchange products. (A) Schematic for experiments with M3′ values of 0, 3, 5. (B) Graphic representation of the change in fluorescence (ΔF) versus time curves from single trial experiments performed with dATP–ssDNA–RecA filaments and DNA Pol IV in which the blue, pink, and light-blue curves correspond to M3′ values of 0, 3 and 5 base mismatches, respectively. ΔF is calculated as the difference between the measured fluorescence and the average initial fluorescence for heterologous dsDNA. (C) Analogous experiments as (B), but with LF-Bsu polymerase instead of DNA Pol IV.