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. 2019 Jan 3;47(4):1977–1986. doi: 10.1093/nar/gky1321

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Single-nucleotide extension defect of pcna-6 (DD41,42AA) at 12°C. Top, substrate and experimental setup are identical to described in Figure 3, except that the 76/29 substrate has a single template adenine residue, and we used full-length Pol δ-DV. Upon addition of dTTP to the preformed complex, extension is by one single nucleotide. Assays were performed at 12°C. Bottom, primer extension is plotted against time in the absence of PCNA (black), with wild-type PCNA (red) or with pcna-6 (blue). The data were modeled to the sum of two exponentials, and k_fast is shown (see Materials and Methods). For the assay without PCNA, the assay was taken out to 1 s, at which time 84% extension had occurred. These data were included in the modelling, but only the portion up to 50 ms is shown.