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A revised model for phage λ Red recombination that includes an interaction with SSB. (1) Redβ (green) is loaded onto the 3′ overhang generated by λ Exo (dsDNA recombination), or binds directly to an electroporated oligonucleotide (ssDNA recombination). (2) The Redβ–ssDNA complex is targeted to the lagging strand of the replication fork through its interaction with SSB (blue). (3) The ssDNA is annealed to the target site as SSB is displaced, until the reaction is complete (4).
