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. 2019 Jan 3;47(4):1964–1976. doi: 10.1093/nar/gky1313

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Growth properties of T. kodakarensis thermosensitive mutants (left) and the complemented strains (right) isolated from the transposon (Tn)-inserted random mutant library. The cells were cultivated in 8 ml MA-YT-Pyr medium in a test tube (n = 3). Left: Growth of tRNA modification mutants at 85°C (A) and 93°C (B), and growth of the complemented strains at 93°C (C). (○) KD2239 control strain; (✦) FFH05 (queE::Tn); (▴) FFH18 (tgtA::Tn); (•); FFH20 (trmI::Tn); (▪)FFH32 (tmcA::Tn); (×) FFH35 (trm11::Tn). Right: Growth of other mutants at 85°C (D) and 93°C (E), and growth of the complemented strains at 93°C (F). (○) KD2239 control strain; (✦) FFH15 (tk0672::Tn); (▴) FFH08 (tk1402::Tn); (•) FFH12 (tk1803::Tn); (▪) FFH27 (tk0647::Tn); (×) FFH22 (tk2145::Tn). In the panels (C) and (F), growth of the strains transformed with the corresponding complementation vector are shown, except for the control strain KD2239.