Table 1.
Transposon insertion sites in T. kodakarensis thermosensitive mutants isolated by random mutagenesis
| Predicted function | Transposon insertion site | Annotation | Mutant strain (direct repeat region [bp no.]) | Complementation |
|---|---|---|---|---|
| DNA topology | tk0470 | Reverse gyrase | FFH11 (1891–1899) | Not tested (8) |
| tRNA modification | tk0754 | tRNA (Met) cytidine acetyltransferase (TmcA) | FFH02, FFH32 (1522–1530) | + |
| tk0760 | 7-Cyano-7-deazaguanine tRNA-ribosyltransferase (TgtA) | FFH16, FFH17, FFH18 (962–970), FFH21 (1167–1175) | + | |
| tk0981 | Guanine10-N2-dimethyltransferase (Trm11) | FFH35 (105–113) | + | |
| tk1198 | Organic radical activating protein (QueE) | FFH05 (595–603), FFH24 (497–505) | + | |
| tk1328 | N 1-methyladenosine methyltransferase (TrmI) | FFH20 (135–143) | + | |
| metabolism | tk0672 | Putative glutamate synthase β-subunit | FFH15 (283–291) | - |
| tk1402 | Polysaccharide deacetylase | FFH08 (526–534) | - | |
| transport | tk1803 | Dipeptide/oligopeptide ABC transporter permease | FFH12 (141–149) | + |
| unknown | tk0647 | Hypothetical protein | FFH27 (582–590) | - |
| tk2145 | Hypothetical protein | FFH22 (518–526) | + |