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. 2019 Feb 21;10:200. doi: 10.3389/fpls.2019.00200

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Copyright © 2019 Li, Wang, Guan, Yang, Wang, Zhang, Ji, Jin and An.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Changes in H₂O₂ (A,C) and O₂^- (B,D) levels in WT and transgenic plants subjected to drought stress. Drought-stressed leaves were incubated in diaminobenzidine (DAB) or nitroblue tetrazolium (NBT) solution. Brown staining indicates H₂O₂ accumulation (A). Blue staining indicates the location and levels of O₂^-(B). H₂O₂ content (C) and O₂^- content (D) were measured after drought treatment. The labels under the graphs correspond to the plant materials shown in (A,B). Control, plants grown under normal conditions; drought, plants grown under drought stress treatment (14 days); WT, wild type. The transgenic lines were named by the construct names followed by the line number: p35S::LcSABP-4 refers to p35S::LcSABP line 4; p35S::LcSABP-8 refers to p35S::LcSABP line 8; p35S::LcSABP-10 refers to p35S::LcSABP line 10. Bars represent the mean ± SE of three independent experiments. ^∗∗Significantly different at the P < 0.01 level compared to WT.