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. 2019 Feb 21;10:178. doi: 10.3389/fpls.2019.00178

FIGURE 2.

FIGURE 2

Overexpression of NtMYB4 significantly reduces salt tolerance. The relative expression levels were normalized to the expression of the Tob103 gene (internal control). The relative expression of NtMYB4 in leaves of WT plants was set to 1. (A) The expression of NtMYB4 in leaves of WT plants was significantly suppressed by the addition of 200 mM NaCl in the medium. Asterisks in (A) indicate significant difference (∗∗P < 0.01) between control condition and salt treatment. (B) The expression level of NtMYB4 in leaves was significantly increased in overexpressing transgenic tobacco compared to the WT. (C,D) The seed germination of WT and three NtMYB4 overexpressing transgenic tobacco lines under 200 mM NaCl. (E,F) The root elongation of WT and three NtMYB4 overexpressing transgenic tobacco lines under 200 mM NaCl. Data are expressed as the mean ± SD as determined from three independent biological replicates. Values in (F) that are significantly different between treatments and among lines within each treatment are marked with ∗∗P < 0.01 and ∗P < 0.05.