FIGURE 4.

Rutin effectively scavenges the ROS levels under salt stress. (A) DAB or NBT staining of WT plants under control (CK) and salt (NaCl) treatment with or without rutin supplementation. (B,C) H₂O₂ or ${\text{O}}_2^{-}$ content of WT plants and NtMYB4 overexpressing transgenic lines under control (CK) and salt (NaCl) treatment with or without rutin supplementation, measured by visible spectrophotometry. FW, fresh weight. Data are expressed as the mean ± SD as determined from three independent biological replicates. Values in (B,C) that are significantly different between treatments and among lines within each treatment are marked with ^∗∗P < 0.01 and ^∗P < 0.05.