FIGURE 7.

NtCHS1 overexpressing transgenic plants significantly increase salt tolerance. (A) The expression level of NtCHS1 in leaves was significantly increased in overexpressing T₀ transgenic tobacco leaves compared to WT. Asterisks in (A) indicate remarkable difference (^∗∗P < 0.01) between NtCHS1 overexpressing transgenic lines and WT. (B) Rutin content increased in the leaves of typical NtCHS1 overexpressing transgenic tobacco lines compared to WT. Asterisks in (B) indicate remarkable difference (^∗P < 0.05) between NtCHS1 overexpressing transgenic lines and WT. (C,D) Root length of WT and NtCHS1 overexpressing transgenic plants (CO-3 line) treated with salt stress. Values in (D) that are significantly different between treatments and among lines within each treatment are marked with ^∗∗P < 0.01 and ^∗P < 0.05. (E,F) H₂O₂ or ${\text{O}}_2^{-}$ content of WT plants and NtCHS1 overexpressing transgenic lines under control (CK) and salt treatment (NaCl), measured by visible spectrophotometry. FW, fresh weight. Data are expressed as the mean ± SD as determined from three independent biological replicates. Values in (E,F) that are significantly different between treatments and among lines within each treatment are marked with ^∗∗P < 0.01 and ^∗P < 0.05.