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. 2019 Feb 21;9:100. doi: 10.3389/fonc.2019.00100

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Copyright © 2019 Ryva, Zhang, Asthana, Wong, Vicioso and Parameswaran.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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WGA binds and kills in a dose- and time-dependent manner. (A) OCI-AML3 cells treated with WGA-FITC (500 ng/mL) and analyzed for binding using flow cytometry. Binding to cells at 45 min (blue peak) and 24 h (red peak) compared to control (black peak). (B) OCI-Ly10 and HL-60 cells treated with WGA-FITC (500 ng/mL) for 30 min and analyzed for binding using flow cytometry. (C,D) Viable cell count and percent viability of HL-60 (C) and (D) OCI-AML3 with WGA treatment (0.5, 1.0, 2.0, and 4.0 μg/mL) for 1–4 days, counted using trypan blue. ^***p < 0.001.