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. 2019 Feb 21;10:137. doi: 10.3389/fphar.2019.00137

FIGURE 3.

FIGURE 3

ZEB1 is a direct target of miR-409-3p in OS. (A) ZEB1 3′ UTR sequences containing wild type and mutant miR-409-3p binding sites. (B) Luciferase reporter assays performed in HEK293T cells co-transfected with miR-409-3p mimics or miR-NC, and pmirGLO-ZEB1-3′UTR Wt or pmirGLO-ZEB1-3′UTR Mut. After transfection (48 h), cells were collected and luciferase activities measured. ZEB1 mRNA (C) and protein (D) were detected in MG63 and HOS cells transfected with miR-409-3p mimics or miR-NC. miR-409, microRNA-409-3p. (E) Protein levels of ZEB1 in tumor tissues and adjacent normal tissues. OS, osteosarcoma. miR-NC, negative control microRNA mimics. Wt, wild type. Mut, mutant. ZEB1, Zinc-finger E-box-binding Homeobox-1. ∗P < 0.05, ∗∗P < 0.01 compared with the control group.