Figure 5.

E. coli fumB and yfbV are not regulated by SdhX due to mutations around their start codons. (A) Difference in fumB-SdhX and yfbV-SdhX interactions between Salmonella and E. coli. The start codon is underlined. Exchanged nucleotides were indicated by red letters. Changes in free energy (ΔG°) upon basepairing are indicated below the interactions. (B) Salmonella ΔsdhX strain was transformed by combinations of mutant pXG plasmids along with pJV300 control vector (null) or SdhX expression plasmid (SdhX). GFP expression was quantified and normalized by OD₆₀₀. Error bars indicate standard deviations (n = 3).