Table 1.
Description of 30 SSR markers used in this study to evaluate the genetic diversity in 292 potato genotypes.
| Code | Name | Map location | Repeat motif | GenBank#, accession# | Primer sequences | Toa | Size (bp) |
|---|---|---|---|---|---|---|---|
| M1 | aSTM1049 | I dg | (ATA)n | X13497 | CTACCAgTTTgTTgATTgTggTg AgggACTTTAATTTgTTggACg |
54 (57) | 197–219 |
| M2 | dSTG0016 | I g | (AGA)n | BI178934 | AgCTgCTCAgCATCAAgAgA ACCACCTCAggCACTTCATC |
55 (53) | 137–174 |
| M3 | dSTM5127 | I eg | (TCT)n | [M23e7] | TTCAAgAATAggCAAAACCA CTTTTTCTgACTgAgTTgCCTC |
55 (60) | 248–291 |
| M4 | aSTM2022 | II deg | (CAA)n…(CAA)n | [C112] | gCgTCAgCgATTTCAgTACTA TTCAgTCAACTCCTgTTgCg |
58 (53) | 173–243 |
| M5 | aSTM1064 | II deg | (TA)n (TG)n GT (TG)n | AC215425 | gTTCTTTTggTggTTTTCCT TTATTTCTCTgTTgTTgCTg |
55 (55) | 201–213 |
| M6 | dSTM5114 | II eg | (ACC)n | [M102B19] | AATggCTCTCTCTgTATgCT gCTgTCCCAACTATCTTTgA |
60 (57) | 297–322 |
| M7 | aSTM1053 | III dg | (TA)n (ATC)n | AB022690 | TCTCCCCATCTTAATgTTTC CAACACAgCATACAgATCATC |
53 (53) | 170–196 |
| M8 | dSTG0010 | III g | (TG)n | BM407152 | CgATCTCTgCTTTgCAggTA gTTCATCACTACCgCCgACT |
60 (55) | 175–192 |
| M9 | eSTM3023 | IV | (GA)9 (GA)8 (GA)4 | AAGCTGTTACTTGATTGCTGCA GTTCTGGCATTTCCATCTAGAGA |
50 | 169–201 | |
| M10 | cSTI0001 | IV fg | (AAT)n | CK860917 | CAgCAAAATCAgAACCCgAT ggATCATCAAATTCACCgCT |
60 (55) | 194–215 |
| M11 | cSTI0012 | IV f | (ATT)n | U69633 | gAAgCgACTTCCAAAATCAgA AAAgggAggAATAgAAACCAAAA |
56 (55) | 183–234 |
| M12 | bSTMoAc58 | V eg | (TA)n | X55749 | TTgATgAAAggAATgCAgCTTgTg ACgTTAAAgAAgTgAgAgTACgAC |
- (57) | 243–263 |
| M13 | cSTI0032 | V fg | (GGA)n | BQ120452 | TgggAAgAATCCTgAAATgg TgCTCTACCAATTAACggCA |
61 (60) | 127–148 |
| M14 | aSTM0019 | VI dg | AT)n (GT)n (AT)n (GT)n (GC)n (GT)n | [MAC33] | AATAggTgTACTgACTCTCAATg TTgAAgTAAAAgTCCTAgTATgTg |
- (47) | 99–206 |
| M15 | cSTI0004 | VI fg | (AAG)n | BQ118939 | GCTgCTAAACACTCAAgCAgAA CAACTACAAgATTCCATCCACAg |
60 (55) | 83–126 |
| M16 | aSTM0031 | VII dg | (AC)n…(AC)n GCAC (AC)n (GCAC)n | [MAC50] | CATACgCACgCACgTACAC TTCAACCTATCATTTTgTgAgTCg |
53 (57) | 185–211 |
| M17 | eSTM2013 | VII | (TCTA)6 | TTCGGAATTACCCTCTGCC AAAAAAAGAACGCGCACG |
55 | 146–172 | |
| M18 | cSTI0033 | VII fg | (AGG)n | BG886969 | TgAgggTTTTCAgAAAgggA CATCCTTgCAACAACCTCCT |
61 (60) | 131–155 |
| M19 | aSTM1104 | VIII deg | (TCT)n | EU548082 | TgATTCTCTTgCCTACTgTAATCg CAAAgTggTgTgAAgCTgTgA |
53 (57) | 178–199 |
| M20 | cSTI0003 | VIII fg | (ACC)n | AW096896 | ACCATCCACCATgTCAATgC CTCATggATggTgTCATTgg |
60 (55) | 137–188 |
| M21 | aSTM3012 | IX d | (CT)n (CT)n | [61D9] | CAACTCAAACCAgAAggCAAA gAgAAATgggCACAAAAAACA |
56 (57) | 180–225 |
| M22 | aSTM1052 | IX eg | (AT)n GT (AT)n (GT)n | AJ133765 | CAATTTCgTTTTTTCATgTgACAC ATggCgTAATTTgATTTAATACgTAA |
50 (52) | 214–263 |
| M23 | cSTI0014 | IX fg | (TGG)n (AGG)n | BQ115461 | AgAAACTgAgTTgTgTTTgggA TCAACAgTCTCAgAAAACCCTCT |
54 (55) | 127–157 |
| M24 | aSTM1106 | X dg | (ATT)n | X95821 | TCCAgCTgATTggTTAggTTg ATgCgAATCTACTCgTCATgg |
51 (55) | 145–211 |
| M25 | dSTG0025 | X g | (AAAC)n | BQ506618 | TggAATCCgAATTACgCTCT AggTTTTACCACTCgggCTT |
56 (55) | 208–223 |
| M26 | dSTG0001 | XI g | (CT)n | BE340539 | CAgCCAACATTTgTACCCCT ACCCCCACTTgCCATATTTT |
58 (52) | 137–163 |
| M27 | aSTM0037 | XI dg | (TC)n (AC)n AA (AC)n (AT)n | [MAC62] | AATTTAACTTAgAAgATTAgTCTC ATTTggTTgggTATgATA |
52 (53) | 87–133 |
| M28 | aSTM0030 | XII deg | comMound(GT/GC) (GT)n | [MAC05] | AgAgATCgATgTAAAACACgT gTggCATTTTgATggATT |
58 (53) | 122–168 |
| M29 | cSTI0030 | XII fg | (ATT)n | BF188393 | TTgACCCTCCAACTATAgATTCTTC TgACAACTTTAAAgCATATgTCAgC |
58 (60) | 94–137 |
| M30 | dSTM5121 | XII g | (TGT)n | [M46L17] | CACCggAATAAgCggATCT TCTTCCCTTCCATTTgTCA |
48 (50) | 297–309 |
The sources of markers were indicated with superscript on top left of marker names as afor Milbourne et al. (1998); bfor Ghislain et al. (2004); cfor Feingold et al. (2005); dfor Ghislain et al. (2009), and efor Ghislain et al. (2006).