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. 2019 Feb 27;9:2905. doi: 10.1038/s41598-019-39427-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Identification of Macrophage Migration Inhibitory Factor (MIF) in GBM patient samples. (A) Flow chart of the steps taken to identify MIF using protein lysates extracted from GBM MGMT methylated patient specimens (n = 36). (B) SELDI-TOF MS spectra demonstrating 3 differentially expressed protein peaks in Group 1 tumors (survival <13.8 months; n = 18) and Group 2 tumors (survival >13.8 months; n = 18). Peaks in red belong to proteins overexpressed in Group 1 tumors. (C) Receiver Operating Characteristic (ROC) analysis for the 3 proteins (S100A8 [blue], DEFA3 [green] and MIF [yellow]) to discriminate Group 1 tumors from Group 2 tumors. The Area Under the Curve (AUC) values are provided. (D) Representative western blots depicting changes in protein expression in lysates from Group 1 (n = 5) and Group 2 (n = 4) tumors. Untreated lysates were extracted from frozen GBM specimens and probed with the indicated antibodies.