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. 2019 Feb 27;9:2905. doi: 10.1038/s41598-019-39427-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunohistochemistry and immunofluorescence analysis of excised mouse brain derived from the in vivo experiment. Brains from the treated mice (n = 8 per treatment group) were harvested at the time of euthanasia. Sections (4 μm) were cut and mounted on ultrafrost slides. H&E, MIF, CD74 and Ki67 proteins were assessed with IHC while Tunel positivity was assessed with immunofluorescence (IF). Representative photomicrographs are presented from each treatment group (i–iv H&E; vii–xii MIF; xiii–xviii CD74; xix–xxiv Ki67 and xxv–xxx Tunel). All images were taken at a magnification of x20 and the error bars represent 100 μm.