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. 2019 Feb 27;9:2856. doi: 10.1038/s41598-019-39076-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fraction of low quality reads visualized as heatmap. The Y-axis represents the individual samples color-coded by library preparation protocol and sequencer model. For each samples the reads are stratified by fragment length and the X-axis indicates the stratum. The R1 reads (left) have a low prevalence of low quality reads while the R2 reads exhibit an increase of low quality reads for fragments longer than 500 nt.