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. 2019 Feb 27;9:2856. doi: 10.1038/s41598-019-39076-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Position specific averaged mismatch rate of short and long fragments in R1 and R2 for all datasets. In R1, the short and long fragments exhibit similar position specific mismatch rate, while in R2, the long fragments lead to much higher mismatch rate than short fragments which even increases towards the end of the read. The smoothing line was generated by “gam” and the confidence interval is 0.99.