Fig. 2.

Exosomes mediate the transfer of circulating myo-miRs into BM-MNCs. Exosomes and the non-exosomal component were isolated from the plasma of AMI and Sham mice 6 h post-surgery. a The levels of myo-miRs in the exosomes and the non-exosomal component were analyzed by qRT-PCR and expressed as fold difference in AMI vs. Sham mice. *p < 0.05, ****p < 0.0001 vs. Sham. n = 5 animals per group. b, c In vitro, freshly-isolated mouse BM-MNCs (2 × 10⁷/well) were treated for 12 h with the exosomes (20 μg) (b) or non-exosomal component (150 μl) (c) from AMI or Sham mice; then, the levels of myo-miRs in the BM-MNCs were quantified with qRT-PCR. **p < 0.01, ****p < 0.0001 vs. treatment with Sham exosomes or Sham non-exosomal component; n.s. not significant. n = 5 biologically independent samples per group. d, e In vivo, exosomes (40 μg in 300 μL PBS/mouse) (d) or the non-exosomal component (300ul/mouse) (e) from AMI or Sham mice was i.v. injected into intact C57BL/6 mice; 12 h later, the levels of myo-miRs in the recipient BM-MNCs were quantified via qRT-PCR. *p < 0.05, **p < 0.01 vs. treatment with Sham exosomes or Sham non-exosomal component; n.s., not significant. n = 5 animals per group. A two-way ANOVA was used for statistical analysis. Error bars represent mean ± s.e.m