Fig. 3.

Myo-miRs downregulate CXCR4 expression in BM-MNCs and MSCs in vitro. BM-MNCs (2 × 10⁶/well) and MSCs (0.5 × 10⁶/well) were transfected with synthesized miR-1a, miR-208a, miR-133a, or miR-499-5p mimics or with a scrambled miR (final concentrations: 50 nM); 24 h later, CXCR4 mRNA expression in the BM-MNCs (a, c, e) and MSC (b, d, f) was quantified by RT-PCR (a, b), Western-blotting (c, d), and flow cytometry (e, f). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 vs. Scrambled. n = 5 biologically independent samples per group. A one-way ANOVA was used for statistical analysis. Error bars represent mean ± s.e.m