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Hydroxytyrosol biosynthesis by pathways 1 and 2. a Optimization of plasmid copy number and gene order for improved hydroxytyrosol production. The time courses of product and intermediate production by the control pathway (containing wild-type HpaBC) (b), pathway 1 (c) and pathway 2 (d) were determined. The data shown are from three replicate experiments and are expressed as the mean ± SD. Source data of a–d are provided in the Excel format Source Data file
