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. 2019 Feb 27;10(3):201. doi: 10.1038/s41419-019-1453-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Representative images of RCC kidney organoid cultures at passage (P) 3, 6 and 9. Microscope magnification ×4. b Percentage of organoid culture formation efficiency starting from normal and RCC fresh tissues. c Kaplan-Meier plot of culture persistence over passages of normal (black) and RCC (red) organoids. d Illustration of the number of cells counted in normal (black) and RCC (red) organoids from passages 6 to 9. Results are expressed as mean ± SD of three independent cultures. e Representative confocal microscope images of normal (upper panel) and tumor (lower panel) organoids stained for Ki67 (green) and actin (red). Nuclei are stained with DAPI (blue). Microscope magnification ×60. Scale bars 50 μm. Immunofluorescence images are representative of at least six independent experiments