Table 1.
Name | 5′ modification | Sequences (5′ to 3′ direction) |
---|---|---|
NDV.A1_PLP | Phosphate | CAATWCCCCCTCTCGCAGTGTATGCAGCTCCTCAGTAATAGTGTCTTACGACGCAACTTCACCGAATGATTCTGGCAAAGCCCCT |
NDV.A2_PLP | Phosphate | YCTGATAGTCTCTCTGTCTTGTGTATGCAGCTCCTCAGTAATAGTGTCTTACGACGCAACTTCACCGAATGATAGATGAAGAAAGTATCTGA |
NDV.A3_PLP | Phosphate | ATGGAGACACTCGATATAGTGTATGCAGCTCCTCAGTAATAGTGTCTTACGACGCAACTTCACCGAATGAAGTCATTTGATATATGGACAT |
NDV.B1_PLP | Phosphate | TGACCCCATTTCCCTGGTGTATGCAGCTCCTCAGTAATAGTGTCTTACGACGCAACTTCACCGAATGAAAGGATATGCTATCYTGAA |
NDV.B2_PLP | Phosphate | RTTAAGACAATACTTTTGCAGTGTATGCAGCTCCTCAGTAATAGTGTCTTACGACGCAACTTCACCGAATGATTGACTGTCTGATATCTCCA |
NDV.B3_PLP | Phosphate | ATTACAGTGTTTTCACGTGTATGCAGCTCCTCAGTAATAGTGTCTTACGACGCAACTTCACCGAATGAGATGCAATGTTGGCACARGAC |
NDV.B4_PLP | Phosphate | TTCTRTTCCGGGCATAATCTGTGTATGCAGCTCCTCAGTAATAGTGTCTTACGACGCAACTTCACCGAATGACCTGTCAAAGGTGACCAGC |
NDV.B5_PLP | Phosphate | RGATATGTGAATGTAAGGTGGTGTATGCAGCTCCTCAGTAATAGTGTCTTACGACGCAACTTCACCGAATGAAATAGCCTTTGRGAATCATT |
NDV.B6_PLP | Phosphate | TTTTTGTTGAGCACGAGTCAGTGTATGCAGCTCCTCAGTAATAGTGTCTTACGACGCAACTTCACCGAATGACCTATGGTTTTCATRTASAA |
NDV.A1 primer | Biotin | GTCCCGAATGACGACATATA |
NDV.A2 primer | Biotin | CATGTCAATCATCTGATAGG |
NDV.A3 primer | Biotin | ACTGCAGGGAATCTCCAACA |
NDV.B1 primer | Biotin | AAGAGTATGYTRGCRATGAG |
NDV.B2 primer | Biotin | AAGAGTATGYTRGCRATGAG |
NDV.B3 primer | Biotin | GTCCTYAAAAAYTCATCTAA |
NDV.B4 primer | Biotin | TYGCRCATGCYATCATGG |
NDV.B5 primer | Biotin | YACRGCTGGGAATCTYCAACA |
NDV.B6 primer | Biotin | TVGGDATTACYAAACTCAAAGA |
Restriction oligo | GTGTATGCAGCTCCTCAGTA | |
Detection oligo | Cy3 | TTTTTGTAAGACACTATTACTGAGG |
Detection oligo* | Cy3 | TTTTTTCATTCGGTGAAGTTGCGTC |
Detection oligo* | Cy5 | TTTTTTATCTCAGCACACGGGACAG |
Detection oligo* | Alexa750N | TTTTTACAGGCAGGCAATCGTTGTA |
W: A or T Y: C or T R: A or G S: C or G V: A or C or G D: A or G or T.
*Detection oligonucleotides used in multiplexing assay.