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. 2019 Feb 27;10(3):207. doi: 10.1038/s41419-019-1384-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — MTT assays were performed to detect the proliferation of AsPc-1 and BxPC-3 cells treated with empty vector, pcDNA-RREB1, pcDNA-RREB1 + si-NC, or pcDNA-RREB1 + si-AGAP2-AS1, and of PANC-1 cells treated with si-NC, si-RREB1, si-RREB1 + empty vector, or si-RREB1 + pcDNA-AGAP2-AS1. b, c Transwell assays were performed with si-AGAP2-AS1-transfected AsPC-1 cells to determine the effects of AGAP2-AS1 on cell migration and invasion. d, e Transwell assays were performed with si-AGAP2-AS1-transfected BxPC-3 cells to determine the effects of AGAP2-AS1 on cell migration and invasion. f, g Transwell assays were performed with pcDNA-AGAP2-treated PANC-1 cells to determine the effects of AGAP2-AS1 on cell migration and invasion. The cells on the underside of the membrane were stained and counted. *P < 0.05, **P < 0.01, ***P < 0.001