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. 2019 Feb 27;9:2950. doi: 10.1038/s41598-019-39588-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of NEMO dynamics. Left panels: normalized B-factors for chains A (a) and B (b) of the structure of NEMO-EEAA. The normalized B-factors are calculated from the average B-factor over all atoms for the residue, and then averaged over a moving window of three residues. Residues 65–92 of chain B are highlighted in green bars. Right panels: Analysis of the dynamics over the last 40 ns of the MD simulation of NEMO-EEAA. The region 65–92 is boxed. (c) Standard deviation of the average interhelical distances calculated as (da + da’)/2, using residue d in the center of each heptad as the abscissa value. (d) RMSF for Cα atoms.