Fig. 3. miR-215 represses invasion and metastasis of PTC both in vitro and in vivo.

a The migration of PTC cells, treated as indicated, was analyzed in wound-healing assays. b The invasive capacities of PTC cells, treated as indicated, were detected by transwell invasion assays. c Representative bioluminescence images of the lung metastasis model. d Representative photographs of dissected lungs from the lung metastasis model. e Number of lung metastatic nodules in the indicated groups (n = 6 per group). f Representative H&E staining of lung metastasis samples (×40). All experiments were performed in triplicate, and the results are presented as the mean ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001