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. 2019 Feb 27;9:2902. doi: 10.1038/s41598-019-39721-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MLKL mediated the necroptosis elicited by P. gingivalis in periodontal ligament fibroblasts. (a) Expression of MLKL and pMLKL in the lysates of PDLFs after P. gingivalis infection (MOI = 400). (b) Effects of NSA on MLKL, pMLKL, RIPK1, RIPK3 and pRIPK3 at 4 h. The images were collected from different gels with the same loading quantity of protein samples in both figures a and b. (c,d) Cell death by release of LDH. (e,f) IL-6 and MCP-1 levels, as shown by ELISA. (g) Cell death after gene knockdown; The images were collected from the same gel. (*p < 0.05; **p < 0.01; ***p < 0.001).