Fig. 6.

The disordered N-terminus of GraT is required to cleave mRNA. Autoradiography of the E. coli lpp mRNA primer extension analysis. MG1655 Δ10TA cultures carrying either the empty vector pBBRlacItac, pBBRlacItac-graTA or pBBRlacItac-Δ22graT were sampled 0, 30 and 60 min after GraT induction at 20 °C (non-induced samples are included as controls). Inducing the full GraT but not the Δ22 mutant variant causes mRNA cleavage. The cut sites follow adenines, usually in the 2nd position of codons. Cleaved codons are shown next to the gel image with cleavage sites, if precisely determined, represented by vertical lines