Fig. 2. AF38469 represses glioblastoma cells migration and invasion in vitro.

a U87 and A172 cell lines were treated with 400 nM sortilin inhibitor (AF38469). Cells were scraped and imaged immediately (0 H) and later (24 H); the images of wound gap were taken for analysis; scale bar = 200 μm. b U87 and A172 cell lines were treated with 400 nM AF38469. Invaded cells were stained and counted using microscopy; scale bar = 200 μm. c U87 and A172 cell lines were transfected with Si-Sor. Invaded cells were stained and counted using microscopy. The histogram corresponds to the mean ± SD of three independent experiment; *p < 0.05, **p < 0.01, scale bar = 200 μm