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. 2019 Feb 27;10(3):208. doi: 10.1038/s41419-019-1449-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, b Western blotting analysis of the expression levels of sortilin, p-GSK-3β, GSK-3β, β-catenin, and Twist in U87 and A172 cell lines treated with 400 nM AF38469 or transfected with Si-Sor. c–f Representative immunofluorescence images to show the expression change of GSK-3β, p-GSK-3β, β-catenin, and Twist in U87 cells; scale bar = 10 μm. Error bars (SD) represent the data of triplicate samples for each marker, paired two-way Student’s t-test; *p < 0.05, **p < 0.01