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. 2019 Feb 27;10(3):208. doi: 10.1038/s41419-019-1449-9

Fig. 6. GSK-3β/β-catenin/Twist is critical for sortilin-stimulated invasion and mesenchymal transition.

Fig. 6

a, b Western blotting analysis of the expression levels of EMT markers (twist, N-cadherin, MMP-9, vimentin, and T-cadherin) in U87 cells treated with SB216763. c, d Western blotting analysis of the activation of GSK-3β/β-catenin/twist in the indicated groups of U87 cells. e, f U87 and 172 cells were treated with AF38469 alone or combined with SB216763. Invaded cells were stained and counted using microscopy. Scale bar = 200 μm. g, h Western blotting to detect the expression levels of EMT markers (twist, N-cadherin, MMP-9, vimentin, and T-cadherin) in U87 cells after overexpressed twist. i, j Transwell assays to detect the invasion ability of U87 cells and 172 cells when treated with AF38469 alone or combined with overexpression of twist. Scale bar = 200 μm. Error bars (SD) represent the data of triplicate samples for each group. Paired two-way Student’s t-test, *p < 0.05, **p < 0.01