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. 2019 Feb 27;9:2863. doi: 10.1038/s41598-019-39239-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Accumulation and size distribution from TYLCV vsRNAs. Tomato plants were infected by either agroinoculation (Agrob-TYLCV) or by B. tabaci (Bemisia-TYLCV). Data from different time points during the systemic infection are shown (7, 14 and 21 dpi). (A) Percentage of vsRNA in the pool of 20–25 nt reads mapped to TYLCV genome relative to the total sRNA reads (19–35 nt). Each bar corresponds to an independent biological replicate. (B) Percentage of each size-class of 20–25 nt TYLCV vsRNA reads (redundant) relative to the total sRNA reads (19–35 nt). The bar from each vsRNA size, corresponds to the mean of the two independent biological replicates. (C) Total number of each size-class in the pool of 20–25 nt unique TYLCV vsRNA reads. The bar from each vsRNA size, corresponds to the mean of the two independent biological replicates.