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. 2019 Feb 27;10(3):197. doi: 10.1038/s41419-019-1458-8

Fig. 5. Inhibition of autophagy blocks the PPARδ-mediated reduction of intracellular lipids and FAO in hepatic cells.

Fig. 5

a PMH were cotreated with 1 mM FFAs (OA:PA = 2:1) and the PPARδ ligand GW0742 (1 μM) ± the lysosomal inhibitor CQ (20 μM) for 24 h. TG content was quantified. b TG content was quantified with or without 10 mM 3-MA pretreatment for 2 h, and three subgroups were analysed after 24 h: blank, 1 mM FFAs (OA:PA = 2:1), and 1 mM FFAs plus 1 μM GW0742. c, e PMH were treated with GW0742 (1 μM) or GW501516 (1 μM) for 24 h in the presence or absence of CQ (20 μM), and then, total RNA was extracted for gene expression analysis. d, f PMH were treated with GW501516 (1 μM) or GW0742 (1 μM) for 24 h in the presence or absence of 3-MA (5 mM), and then, total RNA was extracted for gene expression analysis. Data are expressed as the mean ± SEM of 3 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001