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. 2019 Feb 27;9:2978. doi: 10.1038/s41598-019-39776-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Western blot analysis and 15% SDS-PAGE of purified recombinant proteins using Ni-NTA column chromatography: (a left) Electrophoretic mobility of purified Adnectin C. Lane 1: protein marker, lanes 2, 3: elution 1 and 2 of the purified protein (0.2 mg/ml of purified protein respectively); (a right) Electrophoretic mobility of purified Adnectin C-PAS#1(200). Lane 1: protein marker; lanes 2–4: elution 2–4 of the purified protein (0.1, 0.3 and 1 mg/ml of purified protein respectively). Western blot analysis identified the specified bonds for: (b left) Adnectin C; (b right) Adnectin C-PAS#1(200). The arrows show the band of the expected size of products. Full-length blots and gels are presented in Supplementary Figs S2–S4.