Skip to main content
. 2019 Feb 27;9:2949. doi: 10.1038/s41598-019-39834-7

Figure 2.

Figure 2

DNA fragments amplified from breast tissue samples. DNA quality was firstly analyzed by PCR targeting the GAPDH gene (arrow head, 237 bp) and then submitted to nested PCR targeting BLV Tax gene (open arrow, 113 bp). The gel was loaded using 5 ul of the PCR mix for the GAPDH gene and 5 ul from the nPCR reaction (Lanes 1 to 6). Lanes 1 to 3 depict the results of PCR amplification using DNA from breast cancerous tissue and lanes 4 to 6 represent the result of PCR amplification using DNA from healthy breast tissue. Lanes 7 and 8 contain the fragment amplified from human DNA previously found positive and negative to the presence of BLV DNA, respectively, as indicated in material and methods. Lanes 9 and 10 contain fragments amplified from bovine DNA knowingly positive and negative to BLV. The size of the molecular markers (M) is indicated on the left. The original full-length gel is presented in the Supplementary Information Fig. 1.