Figure 7.

PZ effects in cultures of C. albicans secreting SOD5. C. albicans secreting SOD5 were cultured to mid-log phase as described under “Experimental procedures.” The extracellular growth medium (A) or cells (B) were treated with the indicated concentrations of PZ or DMSO control (0 PZ) for 1 h prior to analysis SOD5 (A) or cell viability (B). A, the growth medium (no cells) was supplemented with 5 μm CuSO₄ for 10 min to activate SOD5 prior to PZ treatment. Medium was analyzed for SOD5 activity by a native gel assay (top), and SOD5 protein levels were analyzed by immunoblotting (bottom) as described under “Experimental procedures.” MW, molecular weight markers (kDa). B, cells were washed with sterile water, and 7000, 700, 70, and 7 cells were spotted on enriched YPD medium. Cells were photographed after 2 days.