Figure 2.
Cytotoxicity of the supernatants from TPG-1–treated RAW264.7 cells to human hepatoma HepG2 and SK-HEP-1 cells. HepG2 (A) or SK-HEP-1 (B) cells were cultured with the supernatants from RAW264.7 cells treated with or without 100 μg/ml TPG-1 for 24 h or fresh complete medium with or without 100 μg/ml TPG-1 for 24, 48, or 72 h, respectively. Cell viability was measured by the MTT assay. The data are representative of three independent experiments, and each was performed at least in triplicate. *, p < 0.05; **, p < 0.01; ***, p < 0.001. Error bars, confidence interval.