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. 2019 Jan 2;294(8):2628–2641. doi: 10.1074/jbc.RA118.005477

Figure 3.

Figure 3.

Effects of TPG-1 on the NO production and iNOS activity in RAW264.7 cells. A, RAW264.7 cells were treated with 0∼500 μg/ml TPG-1 (0, 50, 100, 250, 500 μg/ml TPG-1) for 24, 48, and 72 h, respectively. Cell viability was measured by the MTT assay. B, the level of NO produced by RAW264.7 cells treated with 0∼500 μg/ml TPG-1 for 24 h was examined through a Griess reaction. C and D, total RNA and cell lysates were extracted from RAW264.7 cells treated with 0∼500 μg/ml TPG-1 for 24 h and subjected to quantitative real-time PCR (C) and immunoblotting (D), respectively. The data are representative of three independent experiments, and each was performed at least in triplicate. *, p < 0.05; **, p < 0.01; ***, p < 0.001. Error bars, confidence interval.