Figure 4.
Effects of TPG-1 on the expression of TNFα, IL-6, and COX-2 in RAW264.7 cells. A, C, and E, total RNA were extracted from RAW264.7 cells treated with 0–500 μg/ml TPG-1 for 24 h and subjected to quantitative real-time PCR. B and D, the levels of TNFα (B) or IL-6 (D) in supernatants from RAW264.7 cells treated with 0–250 μg/ml TPG-1 for 24 h were measured by ELISA. F, cell lysates were extracted from RAW264.7 cells treated with 0–500 μg/ml TPG-1 for 24 h and subjected to immunoblotting. The data are representative of three independent experiments, and each was performed at least in triplicate. **, p < 0.01; ***, p < 0.001. Error bars, confidence interval.