Figure 5.
TPG-1 treatment induced TLR4-dependent activation of NF-κB and MAPK signaling in RAW264. 7 cells. A, total RNA was extracted from RAW264.7 cells treated with or without TPG-1 and subjected to RNA sequence, and the scatter plot of differential genes from KEGG enrichment analysis is shown, including the top 30 enriched KEGG pathways. B and C, cell lysates were extracted from RAW264.7 cells treated with 0–500 μg/ml TPG-1 for 48 h and subjected to immunoblotting for the indicated proteins of the NF-κB signaling pathway or MAPK signaling pathway. D and E, cell lysates were extracted from RAW264.7 cells treated with or without 100 μg/ml TPG-1 in the absence or presence of 1 μm TAK-242 for 24 h and subjected to immunoblotting. The data are representative of three independent experiments.