Figure 6.
Up-regulation of TNFα, IL-6, and NO induced by TPG-1 were mediated by TLR4. A–C, the levels of TNFα (A), IL-6 (B), or NO (C) produced by RAW264.7 cells treated with or without 100 μg/ml TPG-1 in the absence or presence of 1 μm TAK-242 for 24 h were measured by ELISA or Griess reaction. D, total RNA were extracted from RAW264.7 cells transfected with TLR4 siRNAs or negative control siRNAs and subjected to quantitative real-time PCR. E, the proliferation of RAW264.7 cells transfected with TLR4 siRNAs or negative control siRNAs were measured by the MTT assay. F–H, RAW264.7 cells transfected with TLR4 siRNAs or negative control siRNAs were treated with or without 100 μg/ml TPG-1 for 24 h, and the levels of TNFα (F), IL-6 (G), or NO (H) in the supernatants were measured by ELISA or Griess reaction. The data are representative of three independent experiments, and each was performed at least in triplicate. **, p < 0.01; ***, p < 0.001. Error bars, confidence interval.