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RPA0376 and RPA2838 have PIMT activity, whereas RPA2580 does not. Methyltransferase activity of R. palustris and E. coli PIMTs. Each reaction contained 0.5 μm enzyme, 10 μm [14C]SAM as the methyl donor, and 560 μm ovalbumin as the methyl acceptor. All the reactions were performed at 30 °C. The error bars represent the standard deviation of at least three replications.
