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The effect of temperature and pH on R. palustris PIMT activities. A and B, the effect of temperature. C and D, the effect of pH. For all experiments, each reaction contained 0.5 μm enzyme, 10 μm [14C]SAM as the methyl donor, and 50 μm VYP-isoD-HA as the methyl acceptor. The error bars represent the standard deviation of at least three replications.
