Figure 6.

R. palustris Δrpa0376 and Δrpa2838 mutants have WT longevity. The PIMT deletion mutants were grown to stationary phase, and then their longevities were measured. The Δrpa0376 and Δrpa2838 mutants maintained ∼100% viability after 20 days of growth arrest. By comparison, the Δrpa2580 mutant showed compromised longevity. Assays performed with double or triple PIMT gene deletions have longevity phenotypes consistent with these observations (Fig. S3). At least three biological replications were performed for each strain (Fig. S5).