Figure 6.

The effect of DPO on VqmA. A, CD spectra of VqmA with no DPO (left) and VqmA with 5 μm DPO (middle). The change trend of the CD spectra results due to the changing temperature at 216 nm (right). B, EMSA for VqmA with no DPO (left) and VqmA with 10 μm DPO (right). C, thermodynamic parameters (ΔG in blue, ΔH in green, −TΔS in red in kJ/mol) determined by ITC for the strong binding of DNA with apo-VqmA and DPO-binding VqmA. D, ITC profiles for the interactions of DNA with apo-VqmA and DPO-binding VqmA.