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. 2018 Dec 28;294(8):2732–2743. doi: 10.1074/jbc.RA118.005079

Figure 4.

Figure 4.

Overexpression of miR-210-5p inhibits the differentiation of anterior hemangioblast to the myeloid progenitors. A–F, whole mount in situ hybridization results showing the expressions of spi1b, tal1, and lmo2 in the 14 hpf embryos microinjected with miR-210-5p mimic or NC. The embryos are positioned dorsally and anterior top. Compared with the embryos microinjected with NC (A), overexpression of miR-210-5p inhibited the expression of spi1b (B), whereas the expressions of tal1 and lmo2 were increased in the ALPM of miR-210-5p overexpressed embryos (D and F) compared with control embryos at 14 hpf (C and E). G, qRT-PCR results showing the reduced expressions of spi1b and increased expressions of tal1 and lmo2 in the miR-210-5p overexpressed embryos at 14 hpf. The gene expression levels in NC group was normalized as 1.0 and the fold-change of gene expression level in the embryos microinjected with miR-210-5p mimic was shown relative to the NC group (shown in y axis). NC, mimic negative control; miR-210-5p, miR-210-5p mimic; *, p < 0.05; **, p < 0.01.