Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Tat-SF1 UHM mediates association with SF3b1 in cell extracts. The amount of transiently expressed FLAG-SF3b1 (143 kDa) retained by immobilized Tat-SF1 proteins (86 kDa; migrates 135 kDa) was detected. Cell lysates (0.5%) were loaded as input controls (left lanes). The sizes of Bio-Rad Precision Plus Dual Color pre-stained markers are indicated (STD). Tat-SF1 (WT) interacts with SF3b1, whereas E286K/F337A mutations (MUT) of the UHM abolished detectable Tat-SF1 association with SF3b1.
