Figure 6.

Overexpression of mGPDH in hepatocytes promotes lower G3P, a reduced mitochondrial NADH/NAD redox state, and increased glycolysis. Mouse hepatocytes were either untreated (Con) or treated with low (L) and high (H) titers of Adv-Gpd2 for overexpression of mGPDH as in Fig. 4. After a 20-h culture to allow protein overexpression, they were incubated for 1 h in MEM containing 25 mm glucose and 0.125 mm octanoate. A, G3P; B and C, 3-hydroxybutyrate/acetoacetate ratio and total 3-hydroxybutyrate + acetoacetate production; D and E, pyruvate and lactate production and lactate/pyruvate ratio; F, cell ATP. Results are means ± S.D. (error bars) for n = 7–11. *, p < 0.05 relative to untreated control.